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Rapley, Ralph:

The Nucleic Acid Protocols Handbook - Taschenbuch

2000, ISBN: 0896038416

[EAN: 9780896038417], Neubuch, [PU: Humana Press], SCIENCE LIFE SCIENCES MOLECULAR BIOLOGY CHROMOSOME DNA ENZYME GENE EXPRESSION GENES HYBRIDIZATION MITOCHONDRIA NUCLEIC ACID PCR RNA SOUT… Mehr…

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Rapley, Ralph:

The Nucleic Acid Protocols Handbook - Erstausgabe

2000, ISBN: 9780896038417

Taschenbuch

[ED: Kartoniert / Broschiert], [PU: Humana Press], Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Part I. Nucleic Acid Extraction. Isolat… Mehr…

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Ralph Rapley:
The Nucleic Acid Protocols Handbook - Taschenbuch

ISBN: 9780896038417

Paperback, [PU: Humana Press Inc.], A comprehensive treasury of the key molecular biology methods - ranging from DNA extraction to gene localization in situ - needed to function effective… Mehr…

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Ralph Rapley:
The Nucleic Acid Protocols Handbook - Taschenbuch

2000, ISBN: 9780896038417

Buch, Softcover, 2000 ed. [PU: Humana Press Inc.], Humana Press Inc., 2000

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Ralph Rapley:
The Nucleic Acid Protocols Handbook - gebrauchtes Buch

ISBN: 9780896038417

Livre, [PU: Humana Press]

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Details zum Buch
The Nucleic Acid Protocols Handbook

A comprehensive treasury of all the key molecular biology methods -- ranging from DNA extraction to gene localization in situ -- needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology "TM" series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps -- all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field.

Detailangaben zum Buch - The Nucleic Acid Protocols Handbook


EAN (ISBN-13): 9780896038417
ISBN (ISBN-10): 0896038416
Taschenbuch
Erscheinungsjahr: 2000
Herausgeber: Humana Press Inc.
1050 Seiten
Gewicht: 1,637 kg
Sprache: eng/Englisch

Buch in der Datenbank seit 2007-11-05T00:35:29+01:00 (Zurich)
Detailseite zuletzt geändert am 2023-03-23T20:49:10+01:00 (Zurich)
ISBN/EAN: 0896038416

ISBN - alternative Schreibweisen:
0-89603-841-6, 978-0-89603-841-7
Alternative Schreibweisen und verwandte Suchbegriffe:
Autor des Buches: walker, jim, rapley
Titel des Buches: acid, handbook


Daten vom Verlag:

Autor/in: Ralph Rapley
Titel: Springer Protocols Handbooks; The Nucleic Acid Protocols Handbook
Verlag: Humana; Humana Press
478 Seiten
Erscheinungsjahr: 2000-03-24
Totowa; NJ; US
Gewicht: 2,138 kg
Sprache: Englisch
219,99 € (DE)

BC; Cell Biology; Hardcover, Softcover / Biologie/Mikrobiologie; Zellbiologie (Zytologie); Verstehen; chromosome; DNA; enzyme; gene; gene expression; genes; hybridization; mitochondria; nucleic acid; PCR; RNA; Southern Blot; tissue; transcription; translation; Cell Biology; BB

Part I. Nucleic Acid Extraction. Isolation of High-Molecular-Weight DNA from Animal Cells, Ian Garner. Isolation of mRNA by Affinity Chromatography, Sian Bryant and David L. Manning. Isolation and Purification of DNA from Plants, Justin Stacey and Peter G. Isaac. Purification of Uncontaminated, Intact Plant RNA, Shu-Hua Cheng, Brandon D. Moore, and Jeffrey R. Seemann. An Improved Method to Isolate Mitochondrial RNA from Green Plant Tissue, Fei Ye and Ralf Reski. Isolating Chromosomal DNA from Bacteria, Elisabeth Chachaty and Patrick Saulnier. Bacterial DNA Extraction for Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis, Elisabeth Chachaty and Patrick Saulnier. Isolation of Fungal Nucleic Acids, Surapareddy Sreenivasaprasad. Total RNA Isolation from Bacteria, John Heptinstall. Simultaneous RNA and DNA Extraction from Biopsy Material, Culture Cells, Plants, and Bacteria, Udo Döbbeling. Spectrophotometric Analysis of Nucleic Acids, John Heptinstall and Ralph Rapley. Part II. Basic Separation and Analysis of DNA. Restriction Endonuclease Digestion of DNA, Duncan R. Smith. Agarose Gel Electrophoresis of Nucleic Acids, D. Ross Williams and Ralph Rapley. Preparation of RNA Dot Blots, Rachel Hodge. Native Polyacrylamide Gel Electrophoresis, Adrian J. Harwood. Southern Blotting of Agarose Gels by Capillary Transfer, Ralph Rapley and Jane Davenport -Jones. Pulsed-Field Gel Electrophoresis, John Maule. HPLC of DNA and PCR Products, Elena D. Katz. Part III. Probe Design, Synthesis, and Labeling. End-Labeling of DNA Fragments, Adrian J. Harwood. Nick Translation and Random Hexamer Labeling of DNA, Jane Davenport-Jones. Generation of Labeled Probes by Polymerase Chain Reaction, Y. M. Dennis Lo and Shu F. An. Nonradioactive Oligonucleotide Probe Labeling, Sue Fowler and Ian Durrant. Preparation of Direct, Enzyme-Labeled DNA Probes, Ian Durrant and Timothy Stone. Random Prime Labeling of DNA Probes with Fluorescein-Tagged Nucleotides, Bronwen M. Harvey, Claire B.Wheeler, and Martin W. Cunningham. Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Chemiluminescence, Claire B. Wheeler, Bronwen M. Harvey, and Martin W. Cunningham. Hybridization of Enzyme-Labeled Probes and Detection by Chemiluminescence, Timothy Stone and Ian Durrant. Hybridization and Competition Hybridization of Southern Blots, Rosemary Kelsell. Autoradiography and Fluorography, Eric Quéméneur. Part IV. RNA Analysis Techniques. Formaldehyde Gel Electrophoresis of Total RNA, Sian Bryant and David L. Manning. RNA Probes for the Analysis of Gene Expression, Dominique Belin. Primer Extension Analysis of mRNA, Maggie Walmsley, Mark Leonard, and Roger Patient. S1 Mapping Using Single-Stranded DNA Probes, Stéphane Viville and Roberto Mantovani. Measurements of Rate of Transcription in Isolated Nuclei by Nuclear 'Run-Off' Assay, Rai Ajit K. Srivastava and Gustav Schonfeld. One-Tube RT-PCR with Sequence-Specific RT Primers, Ulrich Pfeffer and Paola Ferro. Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA, François Mallet. Quantitative Analysis of RNA Species by Polymerase Chain Reaction and Solid-Phase Minisequencing, Anu Suomalainen and Ann-Christine Syvänen. Nonradioactive Northern Blotting of RNA, Rainer Löw. Analysis of RNA by Northern Blotting Using Riboprobes, Rai Ajit K. Srivastava. Part V. Gene Library Construction and Screening. Production of Double-Stranded cDNA for Gene Library Synthesis, Jane Kirk and Steve Mayall. Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs, Yue Zhang and Michael A. Frohman. cDNA Library Construction Using Streptavidin-Paramagnetic Beads and PCR, Kris N. Lambert and Valerie M. Williamson. Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products, Alan R. Shuldiner and Keith Tanner. Subtraction Hybridization cDNA Libraries, Clifford W. Schweinfest, Peter S. Nelson, Michael W. Graber, Rita I. Demopoulos, and Takis S. Papas. Cloning Polymerase Chain Reaction

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