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Rna Interference Screens As A Tool For Discovering Gene Function - Despina Siolas
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Rna Interference Screens As A Tool For Discovering Gene Function - neues Buch

ISBN: 9783843375092

Despina Siolas,Paperback, English-language edition,Pub by AV Akademikerverlag GmbH & Co. KG. Textbooks New Books ~~ Science~~ Life Sciences ~~ Genetics & Genomics RNA-interference-Screens-as-a-Tool-for-Discovering-Gene-Function~~Despina-Siolas AV Akademikerverlag GmbH & Co. KG. Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. Small changes in barcode copy number can be monitored through the use of microarray technology. The barcode can be amplified from a cell's genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies.

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RNA Interference Screens as a Tool for Discovering Gene Function - Despina Siolas
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Despina Siolas:
RNA Interference Screens as a Tool for Discovering Gene Function - Taschenbuch

ISBN: 9783843375092

ID: 594518536

LAP LAMBERT Academic Publishing. Paperback. New. Paperback. 208 pages. Dimensions: 8.7in. x 5.9in. x 0.5in.Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Our second-generation shRNA libraries consist of over 200, 000 short hairpin RNA constructs targeting over 45, 000 human and mouse genes modeled after primary miRNA transcripts. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. Small changes in barcode copy number can be monitored through the use of microarray technology. The barcode can be amplified from a cells genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies. This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN., LAP LAMBERT Academic Publishing

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RNA interference Screens as a Tool for Discovering Gene Function - for cancer genetics studies
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RNA interference Screens as a Tool for Discovering Gene Function - for cancer genetics studies - Taschenbuch

2010, ISBN: 9783843375092

[ED: Taschenbuch / Paperback], [PU: LAP Lambert Academic Publishing], Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. Small changes in barcode copy number can be monitored through the use of microarray technology. The barcode can be amplified from a cell's genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies., [SC: 0.00], Neuware, gewerbliches Angebot, [GW: 295g]

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RNA interference Screens as a Tool for Discovering Gene Function - Despina Siolas
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RNA interference Screens as a Tool for Discovering Gene Function - neues Buch

ISBN: 9783843375092

ID: 2722681ba1a74d2e165605acdd0197ad

Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. Small changes in barcode copy number can be monitored through the use of microarray technology. The barcode can be amplified from a cell's genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies. Bücher / Naturwissenschaften, Medizin, Informatik & Technik / Biologie / Genetik & Gentechnik

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RNA interference Screens as a Tool for Discovering Gene Function - Siolas, Despina
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Siolas, Despina:
RNA interference Screens as a Tool for Discovering Gene Function - Taschenbuch

2010, ISBN: 3843375097

Gebundene Ausgabe, ID: 9816618

for cancer genetics studies - Buch, gebundene Ausgabe, 208 S., Beilagen: Paperback, Erschienen: 2010 LAP Lambert Acad. Publ.

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RNA interference Screens as a Tool for Discovering Gene Function

Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. Small changes in barcode copy number can be monitored through the use of microarray technology. The barcode can be amplified from a cell's genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies.

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EAN (ISBN-13): 9783843375092
ISBN (ISBN-10): 3843375097
Gebundene Ausgabe
Taschenbuch
Erscheinungsjahr: 2010
Herausgeber: LAP Lambert Acad. Publ.
208 Seiten
Gewicht: 0,326 kg
Sprache: eng/Englisch

Buch in der Datenbank seit 14.11.2007 13:53:10
Buch zuletzt gefunden am 07.12.2016 15:25:31
ISBN/EAN: 9783843375092

ISBN - alternative Schreibweisen:
3-8433-7509-7, 978-3-8433-7509-2


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