2010, ISBN: 9783642011436
Springer, Gebundene Ausgabe, Auflage: 2nd ed. 2010, 801 Seiten, Publiziert: 2010-03-26T00:00:01Z, Produktgruppe: Buch, Hersteller-Nr.: 200 black & white illustrations, 20 colo, 3.01 kg, M… Mehr…
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Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity,… Mehr…
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2010, ISBN: 3642011438
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2010, ISBN: 9783642011436
Springer, Gebundene Ausgabe, Auflage: 2nd ed. 2010, 801 Seiten, Publiziert: 2010-03-26T00:00:01Z, Produktgruppe: Buch, Hersteller-Nr.: 200 black & white illustrations, 20 colo, 3.01 kg, M… Mehr…
ISBN: 9783642011436
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity,… Mehr…
ISBN: 9783642011436
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity,… Mehr…
2010, ISBN: 3642011438
[EAN: 9783642011436], Used, very good, [SC: 39.82], [PU: Springer-Verlag Publishing], Very Good++; Hardcover; Close to new condition; Covers are still glossy with "straight" edge-corners;… Mehr…
2010, ISBN: 3642011438
[EAN: 9783642011436], New book, [SC: 11.96], [PU: Springer], Book is in NEW condition., Books
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Detailangaben zum Buch - Antibody Engineering Volume 1 Roland E. Kontermann Editor
EAN (ISBN-13): 9783642011436
ISBN (ISBN-10): 3642011438
Gebundene Ausgabe
Erscheinungsjahr: 2010
Herausgeber: Springer Berlin Heidelberg Core >2 >T
800 Seiten
Gewicht: 1,489 kg
Sprache: ger/Deutsch
Buch in der Datenbank seit 2008-10-01T01:10:35+02:00 (Zurich)
Detailseite zuletzt geändert am 2024-03-05T12:33:27+01:00 (Zurich)
ISBN/EAN: 9783642011436
ISBN - alternative Schreibweisen:
3-642-01143-8, 978-3-642-01143-6
Alternative Schreibweisen und verwandte Suchbegriffe:
Autor des Buches: roland best, kontermann roland stefan dübel, dube, konter
Titel des Buches: vol, springer, engineering antibody volume, engi
Daten vom Verlag:
Autor/in: Roland E. Kontermann; Stefan Dübel
Titel: Springer Protocols Handbooks; Antibody Engineering Volume 1
Verlag: Springer; Springer Berlin
788 Seiten
Erscheinungsjahr: 2010-03-26
Berlin; Heidelberg; DE
Gedruckt / Hergestellt in Deutschland.
Gewicht: 1,364 kg
Sprache: Englisch
353,09 € (DE)
362,99 € (AT)
389,50 CHF (CH)
POD
XIII, 788 p.
BB; Immunology; Hardcover, Softcover / Medizin/Nichtklinische Fächer; Immunologie; Verstehen; Medizin, Gesundheit; Antigen; Cancer therapy; Chloroplast; Expression; Hybridoma; Immunoglobulins; Immunology; Polymerasekettenreaktion; Protein engineering; chromatography; cloning; diagnosis; mutagenesis; protein; transgen; antibodies; Antibodies; Biotechnology; Immunology; Adaptive Immunity; Biotechnology; Biotechnologie; BC; EA; BC
Cloning of the Antigen-binding Site from Hybridoma.- Cloning of Variable Domains from Mouse Hybridoma by PCR.- Coning Hybridoma cDNA by RACE.- Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly.- Generation of Antibody Repertoires.- Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens.- Human Antibody Gene Libraries.- Synthetic Antibody Libraries.- Immune Libraries from Nonhuman Primates (NHP).- Generation of Rabbit Immune Libraries.- Selection of Antibody Fragments from Combinatorial Libraries.- Immunotube Selections.- Phage Display and Selection in Microtitre Plates.- Phage Display and Selections on Biotinylated Antigens.- Phage Display and Subtractive Selection on Cells.- Selection of Phage Antibody Libraries for Binding and Internalization into Mammalian Cells.- Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA.- Yeast Display and Selections.- The Generation of Transgenic Mice Expressing Human Antibody Repertoires.- Selection of Antibody Fragments by Means of the Filter-Sandwich Colony Screening Assay.- Semi-automated Magnetic Bead-Based Antibody Selection from Phage Display Libraries.- Engineering and Production of Immunoglobulins.- Aspects of Isotype Selection.- Generation of Heavy and Light Chains (Chimeric Antibodies).- Humanising Antibodies by CDR Grafting.- Humanization by Resurfacing.- Human Antibodies by Guided Selection.- In Silico De-Immunization.- Affinity Maturation by Chain Shuffling and Site Directed Mutagenesis.- Affinity Maturation by Random Mutagenesis and Phage Display.- Engineering of the Fc Region for Improved PK (FcRn Interaction).- Antibody-Dependent Enzyme Prodrug Therapy (ADEPT).- Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System.- Expression of IgA Molecules in Mammalian Cells.- Antibody Characterization.- Expression of Complete Antibodies in Transgenic Plants.- Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast.- Expression of IgG Antibodies in Mammalian Cells.- Protein A/G Chromatography.- Epitope Analysis Using Synthetic Peptide Repertoires Prepared by SPOT Synthesis Technology.- Epitope Mapping by Printed Peptide Libraries.- Antibody Epitope Mapping Using Yeast Display.- Size Exclusion Chromatography.- Structural Characterization of Antibodies by Mass Spectrometry.- Antibody Glycans Characterization.- Affinity Measurements by Competition ELISA.- Anti-Histidine Antibodies as Tools for Reversible Capturing of His-Tagged Fusion Proteins for Subsequent Binding Analysis.- Affinity Measurements Using Quartz Crystal Microbalance (QCM).- Affinity Measurements with Radiolabeled Antibodies.- Neutralization Tests.- Functional Characterization of Antibodies Neutralizing Soluble Factors In Vitro and In Vivo.- Competitive ELISA.- Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA.- Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay.- Binding Studies with Flow Cytometry.Weitere, andere Bücher, die diesem Buch sehr ähnlich sein könnten:
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